A university official said Saturday that the students have been "temporarily" suspended from classes and will be prohibited from taking their final exams or entering campus, except for access to university housing.
The university also barred 33 other people from entering campus, including students from other colleges and alumni who participated in the protest, the official said.
The decision coincides with negotiations between Columbia University's Board of Trustees and the Trump administration regarding the university's federal funding and independence.
Last Wednesday, a group of students staged a sit-in in the reading room of Columbia University's main library, waving banners denouncing Israel's war of genocide in the Gaza Strip. They were later joined by dozens of protesters who managed to bypass campus security.
The New York Police Department then stormed the campus, at the request of university officials, and arrested dozens of students.
Pro-Palestine demonstrations, which began on several US campuses in April of last year, continued for about six weeks. During this period, protests against Israel and the US administration were held on nearly all major campuses across the country, against the backdrop of the war in the Gaza Strip.
Israel has been strongly attacking American student protests against the war of extermination in Gaza.
The previous US administration, led by Joe Biden, also opposed the protests, but current President Donald Trump announced sanctions against students who participate in the protests.
The US administration is using financial cuts and investigations into universities to pressure university administrations to prevent demonstrations in support of Palestine.